Experimental Developmental Biology: A Laboratory ManualThis work is designed for use as a lab manual in college-level courses in developmental biology or animal development. It could also be used in cell biology lab courses. In each laboratory exercise, students examine gametes and developing embryos of a single species, and also perform several experiments to probe the developmental process best illustrated by that species. A feature of this manual is the preparator's guide, which provides information for setting up each lab. |
Contents
SEA URCHIN FERTILIZATION | 1 |
EXPERIMENTAL PROTOCOL | 4 |
QUESTIONS | 9 |
SUPPLEMENTARY READINGS | 11 |
EARLY AMPHIBIAN DEVELOPMENT | 13 |
EXPERIMENTAL PROTOCOL | 14 |
QUESTIONS | 19 |
SUPPLEMENTARY READINGS | 20 |
SUPPLEMENTARY READINGS | 52 |
Laboratory Requirements | 53 |
WRITING LAB REPORTS | 54 |
Care and Use of Microscopes | 55 |
DETERMINATION OF FIELD DIAMETERS | 56 |
SIZE ESTIMATION | 58 |
DETERMINATION OF VOLUMES | 59 |
Gel Electrophoresis | 61 |
EMBRYONIC CHICK DEVELOPMENT | 21 |
EXPERIMENTAL PROTOCOL | 22 |
QUESTIONS | 30 |
SUPPLEMENTARY READINGS | 31 |
DROSOPHILA GENE EXPRESSION | 33 |
EXPERIMENTAL PROTOCOL | 34 |
QUESTIONS | 40 |
AMPHIBIAN METAMORPHOSIS | 41 |
EXPERIMENTAL PROTOCOL | 42 |
QUESTIONS | 45 |
CELLCELL INTERACTIONS DURING SPONGE AGGREGATION | 47 |
EXPERIMENTAL PROTOCOL | 48 |
QUESTIONS | 51 |
EXERCISES | 62 |
PLASMID MAPPING | 70 |
Guide to Preparation for Laboratory Exercises | 73 |
GUIDE TO PREPARATION LABORATORY EXERCISE 1 SEA URCHIN FERTILIZATION | 76 |
GUIDE TO PREPARATION LABORATORY EXERCISE 2 EARLY AMPHIBIAN DEVELOPMENT | 84 |
GUIDE TO PREPARATION LABORATORY EXERCISE 3 EMBRYONIC CHICK DEVELOPMENT | 89 |
GUIDE TO PREPARATION LABORATORY EXERCISE 4 DROSOPHILA GENE EXPRESSION | 95 |
GUIDE TO PREPARATION LABORATORY EXERCISE 5 AMPHIBIAN METAMORPHOSIS | 99 |
GUIDE TO PREPARATION LABORATORY EXERCISE 6 CELLCELL INTERACTIONS DURING SPONGE AGGREGATION | 101 |
GUIDE TO PREPARATION APPENDIX B MICROSCOPE CARE AND USE | 104 |
GUIDE TO PREPARATION APPENDIX C GEL ELECTROPHORESIS | 106 |
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Other editions - View all
Experimental Developmental Biology: A Laboratory Manual Laura R. Keller,John Hyde Evans,Thomas C. S. Keller No preview available - 1999 |
Common terms and phrases
acid acrylamide actinomycin Agarose Gel Electrophoresis aggregation Amphibian Appendix axolotl beakers blastoderm CaMgFSW cardia bifida casting tray centrifuge cleavage compound microscope coverslip culture dejellying Developmental Biology diameter dissecting microscope Dissolve Drosophila embryo Equipment and Supplies ethidium bromide EXPERIMENTAL PROTOCOL Explantation female fertilization filter paper forceps frog gametes gel apparatus gently Guide to Preparation heat-shock hemacytometer hind-limb II.A II.B II.D II.F incubator ionophore jelly coat Kimwipes lab bench Laboratory Exercise larvae membrane metamorphosis Microscope slides ml distilled H₂O molecular molecular-weight molecular-weight markers multiwell plate NaFSW needle neural Observe orcein P.O. Box Pasteur pipettes petri dish Place plasmid plastic polytene chromosomes power supply Preparation of Lab Prepared slides Remove room temperature salivary glands SDS-PA gel SDS-PAGE sea urchin seawater shell species sperm suspension sponge cells stage stain Steinberg's solution sterile Supplementary Readings tadpoles TAE buffer tanks Telephone thyroxine tissue trypsin tubes unfertilized eggs vitelline water or 20