Fertility CryopreservationRi-Cheng Chian, Patrick Quinn Protecting the reproductive potential of young patients undergoing cancer therapy is increasingly important. With modern treatment protocols, 80% of patients can be expected to survive. It has been estimated that up to one in 250 young adults will be a survivor of childhood cancer in the future; infertility, however, may be a consequence. As a wide range of fertility preservation methods are increasingly offered by clinicians, this systematic and comprehensive textbook dealing with the cryobiology, technology and clinical approach to this therapy will be essential reading to infertility specialists, embryologists, oncologists, cryobiologists, ObGyns, andrologists, and urologists with an interest in fertility preservation. Fertility Cryopreservation reviews all the techniques of this increasingly important field within reproductive medicine. It covers the basic principles of pertinent cryobiology, and contains major sections on the different therapies available, written by international specialists combining experience from both academic centers and commercial industries. |
Contents
1 Cryobiology | 1 |
2 Suppression of ice in aqueous solutions | 10 |
3 Movement of water and cryoprotectants in mouse oocytes and embryos at different stages relevance to cryopreservation | 16 |
4 Cryoprotectants | 24 |
5 Cryopreservation of sperm and testicular tissue | 39 |
6 Sperm cryopreservation for a donor program | 46 |
7 Cryopreservation of surgically retrieved sperm | 51 |
8 Testicular tissue cryopreservation | 57 |
16 Vitrification of human oocytes with different tools | 131 |
17 Vitrification of human oocytes using the McGill Cryoleaf protocol | 144 |
18 Cryopreservation of human oocytes and embryos either by direct plunging into liquid nitrogen or by using an aseptic approach | 157 |
19 Vitrification of human oocytes for a donor program | 169 |
20 Obstetric and perinatal outcomes in pregnancies conceived following oocyte cryopreservation | 178 |
21 Cryopreservation of ovarian tissue | 189 |
22 In vitro culture of human primordial follicles | 200 |
23 Concept of human ovarian tissue | 213 |
9 Cryopreservation of embryos | 67 |
10 Cryopreservation of pronuclear stage human embryos | 76 |
11 Cryopreservation of day two and day three embryos | 89 |
12 Cryopreservation of blastocysts | 95 |
13 Aseptic vitrification of human blastocysts | 106 |
14 Cryopreservation of oocytes | 114 |
15 Cryopreservation of oocytes by slow cooling | 120 |
Common terms and phrases
achieved allow appears assisted blastocysts cancer cells clinical compared concentration considered containing cooling cryo Cryobiology cryoprotectants crystal culture cycle damage disease DMSO donor early effect embryo transfer et al etal ethylene glycol exposure factors Fertil Steril fertility preservation Figure follicles formation freezing fresh frozen frozen–thawed function glycerol graft growth higher Hum Reprod human oocytes implantation important improved increased indicate liquid nitrogen live birth major male maturation medium membrane method mouse obtained oocyte cryopreservation organ outcome ovarian tissue ovary patients performed permeability possible potential pregnancy presence preservation procedure pronuclear protocol recently reduced reported Reprod Biomed Online reproductive retrieval risk samples showed shown slow solution sperm stage stimulation storage straw successful sucrose survival rate technique temperature testicular thawing tion toxicity transplantation treatment vitrification vitro warming women zygotes