Fluorescence Fluctuation Spectroscopy (FFS) Part BThis new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers fluorescence fluctuation spectroscopy and includes chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells.
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Contents
| xiii | |
| xv | |
| 1 | |
| 39 | |
Fluorescence Fluctuation Spectroscopy Approaches to the Study of Receptors in Live Cells | 87 |
Studying the Protein Corona on Nanoparticles by FCS | 115 |
Studying AntibodyAntigen Interactions with Fluorescence Fluctuation Spectroscopy | 139 |
Fluorescence Fluctuation Approaches to the Study of Adhesion and Signaling | 167 |
Interactions in Gene Expression Networks Studied by TwoPhoton Fluorescence Fluctuation Spectroscopy | 203 |
Studying Ion Exchange in Solution and at Biological Membranes by FCS | 231 |
Fluctuation Analysis of Activity Biosensor Images for the Study of Information Flow in Signaling Pathways | 253 |
Probing the Plasma Membrane Organization in Living Cells by Spot Variation Fluorescence Correlation Spectroscopy | 277 |
| 303 | |
| 319 | |
Color Plates | 327 |
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Common terms and phrases
acceptor activity adhesions amplitude analysis antibody average bacterial binding biological biological membranes Biophysical Journal BiophysicalJournal biosensor Cell Biology cell edge cellular ceramide CggR channel Chemistry complex concentration confocal correlation function cross-correlation curves detection determined diffusion coefficient Digman domain donor-only dynamics Edited Eggeling Elson emission Enzyme excitation experimental fFCS Figure filters fluorescence correlation spectroscopy fluorescence fluctuation fluorescence intensity fluorescent proteins fluorophore fraction FRAP FRET FRET–FCS gene Gratton GTPases Horwitz image time series integrin interactions kinetics labeled laser LESTER PACKER VOLUME ligand lipid analogs Lipid rafts living cells measurements methods microscopy molecular brightness molecules Mu¨ller nanoparticles Nienhaus number of molecules observation area ofthe parameters paxillin photobleaching pixel plasma membrane probe proton receptor Rigler sample scanning Schwille signal single-molecule solution spatial spatiotemporal species STED STED-FCS stoichiometry structure subtilis svFCS techniques Tetin trapping two-photon Widengren