Gene Transfer Into Hemopoietic Stem Cells: Prospects for Human Gene Therapy |
Common terms and phrases
ADA-SCID amphotropic assay B9 cells Beusechem bone marrow cells cell line cell types cellen CFU-M CFU-S clonal clones co-culture containing culture detected EC cells ecotropic embryonal carcinoma enhancer experiments expression of hADA factor VIII fibroblasts gene therapy protocols gene transfer genetic genome grafts growth factor hADA-cDNA hematopoietic hemophilia hemopoietic progenitor cells hemopoietic stem cells hemopoietic system hemopoietische human adenosine deaminase hybrid IL-1a and IL-3 infected cells interleukin lethally irradiated mice LgAL(AMo+PyF101 lineage M.P.W. Einerhand Materials and Methods McIvor metrizamide Mo-MuLV murine leukemia virus neoR gene non-adherent cells normal bone marrow packaging cell lines pluripotent pluripotent hemopoietic stem POC-1 cells presence of IL-1a producing cell lines progeny provirus recombinant repopulation retroviral vectors retrovirus packaging cells retrovirus producing cell retrovirus-mediated gene transfer samples sequences Southern blot ẞ-globin supernatants titrated transcription transduced transfer into hemopoietic transplantation U/ml Valerio viral virus production viruses vitro vivo µg/ml