Life Cyle Studies of the T3 Coliphage in Milieus of Singel Carbon-energy Substrates |
Contents
V | 4 |
MATERIALS AND METHODS | 10 |
Determination of Rate of Adsorption | 18 |
1 other sections not shown
Common terms and phrases
15 minutes acetate added to 9.9 adsorbed phage ADSORPTION OF T3 agar layer method agar plate arabinose Assay autoclaved at 15 average number AVERAGE ONE STEP bacterial concentration bacterium broth 5 g burst size cells/ml cellular centrifuge tubes coli CONVERSION EFFICIENCY dicarboxylic acids final titer fumarate give the results glucose glycerol GROWN IN M9-FUMARATE grown in M9-glucose grown in M9-glycerol host cell INFECTED BACTERIA GROWN infected cells intracellular growth Joplik lactate lactose latent period lbs for 15 liberated liter distilled water lysis M9-arabinose M9-lactate M9-lactose M9-malate M9-pyruvate M9-sugars malate ml was added number of virus nutrient agar OBTAINED WITH T3 particles/ml percent adsorption phage more readily phage particles phage production pyruvate rate of adsorption readily than bacteria RELATIVE TITER OBTAINED results obtained rise period STEP GROWTH CURVES step growth experiments substrate succinate synthesis T3 coliphage T3 INFECTED BACTERIA TABLE Titer Time Experiments tryptophane viral virus particles viruses