Medical Biochemistry: Principles and Experiments |
Contents
1 Electrophoresis of Blood Proteins | 3 |
2 Enzymes as Diagnostic Indicators | 16 |
3 Tissue Distribution of Lactate Dehydrogenase Isozymes | 25 |
4 Determination of Glucose in Serum and Urine | 31 |
5 Enzymatic Analysis of Blood Lipids | 37 |
6 The Use of Recombinant DNA in the Detection of Genetic Abnormalities | 47 |
7 Inheritable Diseases and Genetic Engineering | 54 |
8 Use of Radioisotopes in Clinical Biochemistry | 58 |
9 Determination of Glycosylated Hemoglobin | 66 |
10 Biosynthesis of Adrenal Steroid Hormones | 70 |
11 Immunoelectrophoresis of Serum Proteins | 77 |
12 Radioimmunoassay of Thyroxine | 87 |
13 Clinical Analysis of Serum Electrolytes | 92 |
14 LecithinSphingomyelin Ratio of Amniotic Fluid | 98 |
Reagents | 102 |
Common terms and phrases
abnormal absorbance acetate ACTH activity adrenal cortex agar albumin allele amino acid amount Anti Anti antibody antigen antisera assay atomic bicarbonate blood buffer Calculate cells centrifuge CH₂ charge chloride cholesterol Chylomicrons Clinical concentration containing cortisol cuvette dehydrogenase deionized H₂O detected determined diabetes diagnosis Dilute disease Dissolve electron electrophoresis enzymatic enzyme Factor VIII Figure fluid fraction gene genetic globin glucose glycosylated hemoglobin H₂C hexokinase hormone human immunoelectrophoresis immunoglobulin initial velocity ionizable ions isoelectric isoelectric point isotopes isozyme laboratory lactate levels light chain lipids lipoproteins measured metabolic methods mg/dl migrate minutes mixture mole molecular molecules NADH normal ortho-toluidine patient pipette plasma potassium precipitate procedure proteins proton pyruvate radioactive reaction reagent restriction enzyme Sample Worksheet sequence serum sickle-cell sodium solution specific standard steroid strip substrate subunits technetium-99m thyroxine tion tissue triglycerides tube Type urine VLDL