Molecular Testing in Laboratory Medicine: Selections from Clinical Chemistry, 1998-2001, with Annotations and UpdatesDavid E. Bruns, Y. M. Dennis Lo, Carl Wittwer |
Contents
REALTIME PCR AND OTHER HOMOGENEOUS METHODS FOR HUMAN DNA ANALYSIS | 3 |
Simple StartHappy Ending | 18 |
From Living Cells to Microarrays | 28 |
An Update on DzyNAPCR | 35 |
Commentary Rapid Gene Dosage Determination by RealTime Fluorescent PCR | 43 |
CommentaryDNA MethylationResolution in Solution | 61 |
CommentaryFluorescence Polarization Detection in DNA Diagnostics | 70 |
CHIPS AND MICROARRAYS | 77 |
CommentaryNew Rapid DNAbased Tests Will Revolutionize the Practice of Infectious | 217 |
Commentary Molecular Diagnosis of Mycobacteria | 227 |
GENOTYPEPHENOTYPE CORRELATIONS | 235 |
CommentaryMolecular Diagnosis of Intermediate and Severe aAntitrypsin Deficiency by | 250 |
PLASMA DNA AND URINARY | 261 |
CommentaryPresence of Fetal Expanded Dystrophia Myotonica Protein Kinase Alleles | 269 |
Original Article Quantitative Abnormalities of Fetal DNA in Maternal Serum in Preeclampsia | 275 |
Original ArticleDetection of Apoptotic Fetal Cells in Plasma of Pregnant Women | 282 |
Commentary Recent Developments in Microminiaturization for Molecular Diagnostics | 90 |
Experience with SingleNucleotide Polymorphisms Neural | 104 |
Emerging Techniques for DNA Extraction | 112 |
Original Article Genotyping EnergyTransferCassettelabeled ShortTandemRepeat Amplicons | 129 |
MASS SPECTROMETRIC METHODS IN INBORN ERRORS OF METABOLISM | 137 |
Commentary Determination of Point Mutations or SingleNucleotide Polymorphisms by Matrixassisted | 143 |
CommentaryOn the Joys of Combining Pediatric Clinical Chemistry and Research on Inborn | 152 |
CommentaryHPLCElectrospray Tandem Mass Spectrometry in Screening for Disorders | 161 |
Commentary From MALDITOF MS to TwoDimensional CEFTICRMS of Proteins in Spinal Fluid | 171 |
INFECTIOUS DISEASE | 201 |
CommentaryUse of Microchip Electrophoresis in Clinical Assays for Virus Detection | 209 |
Original ArticlePlasma DNA as a Prognostic Marker in Trauma Patients | 287 |
Original Article Presence of Donor and Recipientderived DNA in Cellfree Urine Samples of Renal | 294 |
Possible Universal Method for Early Tumor Detection | 300 |
Technical Aspects Semantics | 309 |
PLASMA | 317 |
Original ArticlePresence of Fetal RNA in Maternal Plasma | 323 |
PHARMACOGENETICS | 331 |
The Yin and Yang of Genotyping and Phenotyping | 343 |
| 355 | |
Common terms and phrases
acylcarnitines allele allele-specific amplicons Anal Chem analysis analyzed array ATCC B-globin cancer capillary capillary array cell-free cells chip circulating Clin Chem Clinical Chemistry Clinical Chemistry 47 cycles deficiency disease disorders DNA concentrations DNA fragment DNA in maternal DNA sequencing exon ferritin fetal DNA filter fluorescence gene genetic genomic genomic DNA genotyping hemochromatosis heterozygous homozygous hybridization probes identified increased laboratory LightCycler MALDI-TOF markers mass spectrometry maternal plasma matrix-assisted laser desorption/ionization melting curves metabolic method methylation microchip microchip electrophoresis mismatches mmol/L molecular beacons Molecular Diagnostics MS/MS multiplex newborn screening nucleic acid nucleotide obtained oligonucleotide patients PCR amplification PCR assay PCR products peaks performed plasma plasma DNA polymerase polymorphism postmortem primers profiles protein rapid ratio reaction sensitivity separation serum signal specific TaqMan target temperature tion TPMT tumor urinary DNA urine samples wild-type