Monoclonal Hybridoma Antibodies Techqs & ApplnsJohn G. R. Hurrell The first section of this volume is aimed to provide a comprehensive review of the many varied and often empirically derived techniques and procedures currently in use to produce monoclonal hybridoma cell lines and to characterize the antibodies secreted. The goal has been achieved with the chapter contributed by Zola and Brookes who, as each step in the process of hybridoma production and antibody characterisation is reviewed, have provided an experimental procedure found to be satisfactory in their laboratory.The second section of this volume is designed to provide a review of areas in which monoclonal hybridoma antibodies have been of particular advantage. This is a rapidly advancing field which could not be thoroughly reviewed in a single volume. |
Contents
Chapter | 4 |
II | 5 |
Techniques for the Production and Characterization of Monoclonal | 16 |
Copyright | |
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activity affinity alloantisera amino acid analysis anti antigenic determinants antigenic drift antigenic variants antisera assay BALB/c binding carcinoembryonic antigen carcinoma cell lines cell surface clonal antibodies clones complex conventional antisera cross-reactions culture cytotoxic daltons defined detected dilution electrophoretic epitopes feeder cells fused fusion genes glycoprotein gC glycoproteins growth hormone hemagglutinin herpes simplex virus human hybridoma hybridoma antibodies immune immunofluorescence immunoglobulin Immunol immunologic immunoprecipitation infected cells influenza virus labeled laboratory leukemia lymphocytes lymphoid MAbs macrophages medium melanoma melanoma cells membrane method mice mol wt molecular weight molecule monoclonal antibodies monomorphic mouse murine myeloma cells myeloma line myocardial infarction Natl Nature London neuraminidase normal parasite patients peptide polypeptide precipitated Proc produced protein purified radioimmunoassay react reaction reactivity screening selected sequence sera serum specific spleen spleen cells strains structure studies technique tested thymocytes tibodies tissue titer viruses