The Biosynthesis of Prostaglandins |
Contents
Cofactor Requirement | 6 |
Enzyme Purification | 14 |
Protein Determination | 18 |
5 other sections not shown
Common terms and phrases
14-eicosatrienoic acid 2,7-dihydroxynaphthalene 30 minutes 8,11,14-eicosatrienoic acid Addition PGA2 adrenochrome assay agents on prostaglandin arachidonic acid assay conditions bovine seminal vesicle buffer chelate cofactors COOH described in Methods deuterated and non-deuterated deuterium effect of glutathione eicosatrienoic eicosatrienoic acid elution enzyme ethyl acetate fatty acids Figure flufenamic acid fraction glandin synthetase activity glutathione hydrogen abstraction hydroquinone hydroxyl ibuprofen incubated for 30 incubation and assay indomethacin inhibited prostaglandin isotope effect isotope enrichment k₂ kinetic isotope enrichment L-epinephrine lipoxidase mechanism mefenamic acid metal chelates Microsomes minutes at 37 mixture 10 ml ml aliquots naproxen oxidation oxygen PGD 2 PGE2 PGD2 PGE2 PGE2 and PGF pH 9 Preincubation presence prosta prostaglandin formation prostaglandin synthetase activity protein purification radioactive assay ratio reducing equivalents seminal vesicle seminal vesicle homogenates Serotonin shows specificity of GSH substrate supernatant superoxide anion superoxide dismutase Takeguchi thiols tions utilized Zimmerman assay он