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Acad ACE1 ACE2 activates transcription activity of HSF antioxidant antioxidant activity Biol carbon source cloning containing copper MT CUP1 expression CUP1 gene CUP1 mRNA levels CUP1 promoter CuZnSOD D. J. Thiele data not shown demonstrate DNA binding activity DNase DNase I footprinting domain of HSF eukaryotes expression during oxidative fragment function gene expression genetic glucose glucose starvation growth defects heat shock factor heat shock induction heat shock transcription heat shock treatment HSF binding HSF1 gene HSF2 HSP70 gene induction of CUP1 Isogenic Karin lactate metallothionein metallothionein gene expression methylation interference mutant cells Natl oxidative stress pathway phosphorylation phosphorylation of HSF plasmid response role Saccharomyces cerevisiae sequence shock transcription factor signal transduction SNF1 SNF1 and SNF4 SNF1 gene SNF1 protein kinase snfl mutant sodlA strains Southern blot SSA3 genes subcloning superoxide dismutase transcription during oxidative transcription factor transcriptional activation uracil vitro wild type yeast