Novel Anticancer Drug Protocols (Google eBook)

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John K. Buolamwini, Alex A. Adjei
Springer Science & Business Media, Jan 1, 2003 - Medical - 372 pages
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This book not only surveys the whole arena of novel anticancer drug targets, but also presents a wide-ranging selection of cuttingedge techniques currently being applied throughout novel antitumor drug discovery and development. The methods are applied to experiments involving such topics as immunotherapy, angiogenesis, cancer metastasis, the cell cycle, signal transduction inhibitors, apoptosis, antibodies, antisense molecules, microarray gene expression, flow cytometry, and PET imaging for cancer target validation. There are also proven methods for the preclinical identification of drug targets. Validate new drug targets both preclinically and clinically Identify novel anticancer drug targets in preclinical stages Follow proven instructions to obtain successful results and avoid failure.
  

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Contents

Overview of Novel Anticancer Drug Targets
3
Kinase Inhibitor Discovery Protocols
29
Biomarker Assays for Phosphorylated MAP Kinase Their Utility for Measurement of MEK Inhibition
31
Assays for CyclinDependent Kinase Inhibitors
39
Identifying Inhibitors of ATM and ATR Kinase Activities
49
Angiogenesis and Metastasis Protocols
57
The Rat Aortic Ring Assay for In Vitro Study of Angiogenesis
59
Real Time In Vivo Quantitation of Tumor Angiogenesis
65
ProteinProtein and ProteinDNA Interactions
173
The Mammalian TwoHybrid Assay for Detection of CoactivatorNuclear Receptor Interactions
175
Preparation of DNAProtein Complexes Suitable for Spectroscopic Analysis
185
Antisense
203
Antisense Oligonucleotide Inhibitors of MDM2 Oncogene Expression
205
Genomics
223
Pharmacogenetic Analysis of Clinically Relevant Genetic Polymorphisms
225
Gene Expression Microarrays
239

Use of TumorActivated Hepatic Stellate Cell as a Target for the Preclinical Testing of AntiAngiogenic Drugs Against Hepatic Tumor Development
79
Cell Motility Adhesion Homing and Migration Assays in the Studies of Tyrosine Kinases
87
Inflammatory Response of TumorActivated Hepatic Sinusoidal Endothelium as a Target for the Screening of Metastasis Chemopreventive Drugs
107
Binding Assay for Selectins
117
Determination of Peak Serum Levels and Immune Response to the Humanized AntiGanglioside AntibodyInterleukin2 Immunocytokine
123
Immunohistochemical Assays in the Clinical Setting
133
Immunohistochemical Determination of EGFRTyrosine Kinase Inhibition in Clinical Samples
135
Immunohistochemical Assays of Farnesyltransferase Inhibition in Patient Samples
141
Protein ChaperoningDegradation Protocols
147
Assays for HSP90 and Inhibitors
149
Assays for Proteasome Inhibition
163
Methods for Isolation and Genetic Analysis of Circulating Tumor DNA in Patient Plasma
257
Cell LifespanLongevity
263
The Use of Early Sea Urchin Embryos in Anticancer Drug Testing
265
In Vivo Imaging
277
PET Screening of Anticancer Drugs A Faster Route to DrugTarget Evaluations in Vivo
279
Miscellaneous Protocols
295
Assays for In Vitro and In Vivo Synergy
297
Flow Cytometric Methods for Detection and Quantification of Apoptosis
323
Assays for Neoplastic Cell Enrichment in Bone Marrow Samples
333
Index
343
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