Methods in Cancer ResearchHarris Busch |
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Page 123
... activity . When the mRNA was methyl- ated , protein synthesis was markedly stimulated . S - Adenosylhomocysteine ... activity of the system . Muthu- krishnan et al . ( 1975 ) showed that removal of the terminal nucleotide m'G by ẞ ...
... activity . When the mRNA was methyl- ated , protein synthesis was markedly stimulated . S - Adenosylhomocysteine ... activity of the system . Muthu- krishnan et al . ( 1975 ) showed that removal of the terminal nucleotide m'G by ẞ ...
Page 138
... activity was eluted in the concentration range of 0.2-0.4 M NaCl . When these fractions were combined , dialyzed against distilled water , and lyophilized , 42,000 units were recovered from 50 livers with a specific activity of 200 U ...
... activity was eluted in the concentration range of 0.2-0.4 M NaCl . When these fractions were combined , dialyzed against distilled water , and lyophilized , 42,000 units were recovered from 50 livers with a specific activity of 200 U ...
Page 202
... activity of dihydro- folate reductase is a direct spectrophotometric assay in which the dihydrofolate - dependent disappearance of TPNH is measured at 340 nm ( Osborn and Huennekens , 1958 ) . This procedure is generally applicable to ...
... activity of dihydro- folate reductase is a direct spectrophotometric assay in which the dihydrofolate - dependent disappearance of TPNH is measured at 340 nm ( Osborn and Huennekens , 1958 ) . This procedure is generally applicable to ...
Contents
CHAPTER I | 1 |
Wilms Tumor Wataru W Sutow I Introduction II Clinical Aspects III Treatment IV National Wilms Tumor Study V Miscellaneous Considerations Re... | 29 |
Receptors | 67 |
Copyright | |
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Acad acid activity added addition antibody antigen approach assay assumptions binding Biochem Biol bound buffer Cancer centrifuged challenge Chem chromatography column complex concentration containing curves cytoplasmic damping demonstrated described determined developed dihydrofolate reductases diploid cells effect enzyme et al experimental experiments extraction fraction function glucocorticoid growth hormone hosts immunity increase incubation indicated induced inhibition initial inoculation isolation labeled lines liver MCA-F mean measured method mice minutes mixture mRNA muscle Nature observed obtained patients period polysomes population prepared present Proc procedure proportion protein reaction recently receptor relative reported resistance response sensitive showed similar soluble solution specific steroid studies suggested synthesis Table tetraploid cells tissues translation transplantation treated treatment TSTA tumor cells values vincristine vitro volume