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7.4 phosphate buffer absorbance Acta Aldrich aliquots of nitric Barker Biochem biosensors buffer solution calibrations with different Chem Chromatium Chromatium vinosum chromoionophore colloidal gold concentrations of nitric Cusanovich determined deviations are based different sensors dye-labeled cytochrome emission excitation fiber optic sensors Figure fluorescein fluorescein derivative fluorescence intensity fluorescence lifetime glutathione gold colloid guanylate cyclase heme domain hemoprotein hydrogen peroxide Immunol intracellular ionophore Kopelman labeling dye laser limit of detection lipopolysaccharide LPS and IFN-y macrophage nitric oxide macrophages macrophages activated Marletta measurement number Molecular Probes multiple calibrations nano-optodes nanosensor nitric oxide concentration nitric oxide levels nitric oxide production nsec optical fiber optodes Oregon Green oxide in 100 peroxynitrite pH 7.4 phosphate photobleaching prepared with dye-labeled protein purged with N2 purpureus cytochrome ratio ratiometric sensor Ro/R Sensor calibration sensor response Shortreed silanized Solid line spectra Standard deviations Stuehr succinimidyl ester superoxide Taha taken in 100 vinosum cytochrome