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absence of coal acetate acetogenic acids and alcohols active coal degrading agitation anaerobic analysis Appendix bacterial basal salts medium benzyl benzoate BESA biogasification consortium biogasification experiments biogasification of Texas biomass biomass concentration bottles calibration chemical oxygen demand cinnamic acid coal biogasification coal conversion coal particle coal to methane containing Control dithiothreitol enrichment gas chromatograph gas sample growth headspace headspace gas inoculated inoculum isolate lignite by Mic-1 liquid samples low-rank coals mesh metabolism Methanococcus methanogens Methanosarcina Methanosarcina sp Methanothrix sp method Mic-1 and Mic-4 Mic-1 consortium Mic-1 culture microbial populations micronized coal microorganisms microscopic Model Compound Culture monensin monitored present primary coal degrading Production by Mic-1 production of methane protein protein assay protein concentration rate of methane reaction mixtures reagent redox potential rods Sodium Benzoate Sodium sulfide standard substrates temperature termite termite medium Texas lignite total gas transfer tubes VFAs volatile fatty acids volume weeks of incubation