Production of Membrane Proteins: Strategies for Expression and Isolation (Google eBook)
Anne Skaja Robinson
John Wiley & Sons, Jun 15, 2011 - Science - 432 pages
Designed as a research-level guide to current strategies andmethods of membrane protein production on the small to intermediatescale, this practice-oriented book provides detailed, step-by-steplaboratory protocols as well as an explanation of the principlesbehind each method, together with a discussion of its relativeadvantages and disadvantages.
Following an introductory section on current challenges in membraneprotein production, the book goes on to look at expression systems,emerging methods and approaches, and protein specificconsiderations.
Case studies illustrate how to select or sample the optimalproduction system for any desired membrane protein, saving bothtime and money on the laboratory as well as the technicalproduction scale.
Unique in its coverage of "difficult" proteins with largemembrane-embedded domains, proteins from extremophiles, peripheralmembrane proteins, and protein fragments.
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Membrane Protein Production Using
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