Production of Membrane Proteins: Strategies for Expression and Isolation (Google eBook)
Anne Skaja Robinson
John Wiley & Sons, Jun 15, 2011 - Science - 432 pages
Designed as a research-level guide to current strategies and methods of membrane protein production on the small to intermediate scale, this practice-oriented book provides detailed, step-by-step laboratory protocols as well as an explanation of the principles behind each method, together with a discussion of its relative advantages and disadvantages.
Following an introductory section on current challenges in membrane protein production, the book goes on to look at expression systems, emerging methods and approaches, and protein specific considerations.
Case studies illustrate how to select or sample the optimal production system for any desired membrane protein, saving both time and money on the laboratory as well as the technical production scale.
Unique in its coverage of "difficult" proteins with large membrane-embedded domains, proteins from extremophiles, peripheral membrane proteins, and protein fragments.
What people are saying - Write a review
We haven't found any reviews in the usual places.
Expression of GProteinCoupled Receptors
Structural Biology of Membrane Proteins
Expression and Purification of GProtein
Stabilizing Membrane Proteins in Detergent
Rapid Optimization of Membrane Protein
Membrane Protein Production Using
Acad Acta activity affinity amino acid assay bacterial baculovirus bicelles Biochem Biochemistry Biol Biophys Biotechnol bovine buffer C-terminal cannabinoid cell lines cell-free channel characterization Chem chromatography cloning concentration coupled receptors Crystal structure culture CXCR1 detergent detergent micelles domain enzyme Escherichia coli eukaryotic expression levels expression system fluorescence folding G protein-coupled receptor G-protein gene glycosylation GPCRs H3 receptor heterologous expression hydrophobic IMPs increase insect cells integral membrane proteins interactions ligand ligand binding lipid bilayer liposomes mammalian cells membrane protein expression method micelles molecular mutants Natl neurotensin neurotensin receptor NTR1 optimal overexpression peptide phospholipid Pichia pastoris plasma membrane plasmid pmol/mg Proc protease Protein Expr Protein Sci protein-coupled receptor purification receptor expressed recombinant proteins reconstitution refolding residues rhodopsin Saccharomyces cerevisiae sample screening selection sequence signal solid-state NMR solubilization solution spectroscopy stability strains strategy structural studies target protein transmembrane vectors virus wild-type yeast yield