Production of Membrane Proteins: Strategies for Expression and Isolation (Google eBook)

Front Cover
Anne Skaja Robinson
John Wiley & Sons, Jun 15, 2011 - Science - 432 pages
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Designed as a research-level guide to current strategies andmethods of membrane protein production on the small to intermediatescale, this practice-oriented book provides detailed, step-by-steplaboratory protocols as well as an explanation of the principlesbehind each method, together with a discussion of its relativeadvantages and disadvantages.
Following an introductory section on current challenges in membraneprotein production, the book goes on to look at expression systems,emerging methods and approaches, and protein specificconsiderations.
Case studies illustrate how to select or sample the optimalproduction system for any desired membrane protein, saving bothtime and money on the laboratory as well as the technicalproduction scale.
Unique in its coverage of "difficult" proteins with largemembrane-embedded domains, proteins from extremophiles, peripheralmembrane proteins, and protein fragments.
  

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Contents

List of Contributors
Introduction
Expression Systems
Strains
Membrane Protein Expression in Saccharomyces
Pichia pastoris
Heterologous Production of Active Mammalian
Membrane Protein Expression in Mammalian Cells
Expression of GProteinCoupled Receptors
Structural Biology of Membrane Proteins
Emerging Methods
Expression and Purification of GProtein
Acknowledgments
Stabilizing Membrane Proteins in Detergent
Rapid Optimization of Membrane Protein
Acknowledgments

Membrane Protein Production Using
ProteinSpecific

Common terms and phrases

About the author (2011)

Anne Skaja Robinson is Professor and Associate Chair forBiochemical Engineering in the Department of Chemical Engineeringat the University of Delaware in Newark, Delaware, USA. Sheobtained her Bachelor's and Master's degrees from The Johns HopkinsUniversity, and her Ph.D. from the University of Illinois atUrbana-Champaign. Prior to joining the faculty at Delaware in 1997,she was a postdoctoral fellow in the Department of Biology at MIT.She is a widely acknowledged leader in the field of proteinproduction, and has authored over 50 scientific publications. Also,she is the recipient of numerous awards, including NSF'sPECASE/Career award, the DuPont Young Professor award, and is an2011 AIMBE Fellow. She is a member of the Editorial Boards ofBiotechnology and Bioengineering, and Biotechnology Journal, andthe former Chair of the American Chemical Society BIOTdivision.

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