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Modulation of Promoter Escape by Sigma-core Interactions of the Bacterial ...
Mark Edward Leibman
No preview available - 2007
1 Structure of a transcription elongation complex TEC
The transcription cycle
32 other sections not shown
active antitermination Artsimovitch and Landick assay average Bacillus subtilis backtracking bacterial binding Chamberlin ChlP ChlP-chip clamp helices coli RNA polymerase concentration of o70 concentration of tethered contacts containing core RNAP crosslinking Darst detected downstream effective concentration elongation factor elongation rate encoding enhancement enzyme Escherichia coli Escherichia coli RNA factor NusG Figure function gene hairpin heat shock Hippel holoenzyme individual domains initiation interaction with RNAP IPTG linker Materials and methods modulate Mol Biol Mooney and Landick Murakami mutant nascent RNA Nehrke Nudler NusA NusA and NusG NusG NusG protein NusG/RNAP o70-dependent pausing operons overexpression p-dependent termination Pasman paused TEC plasmid promoter-proximal purified rebinding region RfaH RNA chain RNA polymerase RNAP signal sigma 70 sigma factor stimulate stochastic release strain structural domains subunit TECs template termination factor tethered o70 transcription factor transcription termination transcription units untethered o70 vitro vitro transcription vivo wild-type wild-type RNAP