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added to 500 adjusted to 7*0 aerobic agar gel agar liquefaction Agar Liquefying Bacterium agar plates Agnes Allison Nichols ammonium sulphate appears autoolaved Bacillus gelatious bacteria bouillon butter muslin carbohydrates carbonate cell cellu cellulose agar cellulose fermentors cellulose medium cellulose peptone agar cellulose suspension Characteristics Cheltenham citrate College of Agriculture colonies concentrated sulphuric acid Cornell University Cultures carried curd Dairy deep pitting deep yellow demonstrated deoantation diameter distilled water pH fermenting agar fermentors of milk filter paper firm edged flagella formed Further attempts Gelatine Gran growth and agar growth with pit heavily inoculated ingredients isolated lactose laotate lose medium prepared medium show nutrient agar nutrient broth nutrient solution obtained organism destroying pellicle practically no growth pure culture shaking the tubes simple agar simple medium slants sodium soil plates soil sample source of nitrogen source of oarbon starch streaked sucrose surface three weeks utilize cellulose Vibrio yellow clouding yellow growth