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Methioninelnduced Stabilization of BowmanBirk Protease
BowmanBirk Protease Inhibitor mRNA Degradation with
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50 mM Tris-CI amino acids B-conglycinin B-subunit basal medium basal-cultured cotyledons BBPI mRNA degradation BBPI mRNA levels BBPI mRNA stability BBPI mRNAs Biermann c-mvc mRNA cap structure cDNA cDNA clone 6-7 cell-free mRNA degradation cell-free system cis-acting elements cordycepin cultured cotyledons cultured in basal cultured soybean cotyledons degradation intermediates EDTA endonucleolytic cleavage exonucleases fraction from basal-cultured fraction from met-cultured gene expression glycinin half-life half-life of BBPI Higgins Holowach hybridization incubated inhibited Kunitz inhibitor legumin and albumin levels of BBPI met-cultured cotyledons methionine Methods section Mg++ MgCI2 Mol Cell Biol mRNA degradation system mRNA degradative activity nucleotide pea seeds Plant Physiol poly poly(A polysomal polysomal and postpolysomal polysomal fraction postpolysomal fraction probe protease inhibitors regulation ribosomal Ross S-ethyl-cysteine S1 nuclease S1 nuclease analysis sequence shown Southern blots soybean cotyledons cultured soybean genome soybean seeds stability of BBPI stem-loop stem-loop structure sulfur sulfur amino acids sulfur-deficient conditions Tan-Wilson Tris-CI pH 7.5 vitro