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acetate agar agglutination albumin amino acids amino sugars amount antibody antigen antiserum applied approximately assay bacterial Biochem Büchner funnel buffered saline carbohydrates cell suspension centrifuge tubes Chem chromatographic cold room column complement components concentration containing described in Exercise detection determination dialysis dilution Dissolve electrophoresis endotoxic endotoxin enzyme erythrocytes esters Evaluation extract flask fractions H2SO hemagglutination hemolysis hexane human serum hydrolysis immunoelectrophoresis immunoglobulin Incubate injection layer lipids lipopolysaccharide Materials and Equipment measured membrane methanol method methyl mg/ml mixture ml distilled water ml saline ml water NaOH Nowotny obtained oligosaccharides OO ml paper chromatography pipette plates polysaccharide precipitate preparation procedure protein quantitative reaction reagent red blood cells remove room temperature saline sample sediment separation silicic acid slides sodium solution solvent spleen SRBC staining standard sterile stirring supernatant supernate test tubes tion vacuum wash