Genetic Characterization of Wheat Germplasm with Resistance to Fusarium Head Blight (FHB) and Powdery Mildew

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ProQuest, 2007 - 200 pages
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A dominant powdery mildew resistance gene transferred to the hexaploid germplasm line NC99BGTAG11 from T. timopheevii subsp. armeniacum was mapped distally on the long arm of chromosome 7A. Differential reactions were observed between the resistance gene in NC99BGTAG11, the resistance gene in NC96BGTA4, and the alleles of the Pm1 locus that are also located on chromosome arm 7AL. Observed segregation in F2:3 lines from the cross NC99BGTAG11 x Axminster (Pm1a) and from the cross NC99BGTAG11 x NC96BGTA4 demonstrate that germplasm line NC99BGTAG11 carries a novel powdery mildew resistance gene, which is now designated as Pm37. Analyses of the population with molecular markers indicate that Pm37 is located 16 cM proximal to the Pm1 complex. However, further identification of linked markers is necessary to resolve the location of the resistance gene in NC96BGTA4. Simple sequence repeat (SSR) markers Xgwm332 and Xwmc790 were located 0.5 cM proximal and distal, respectively, to Pm37. Two new EST-derived STS markers were located distal to Pm37 and one marker was closely linked to the Pm1a region.
  

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Contents

Breeding for wheat resistance to fungal diseases
11
MarkerAssisted Selection MAS
34
Objectives
41
Pm37 a new broadly effective powdery mildew resistance gene
77
Materials and methods
83
Results
90
References
99
Haplotype structure and genetic diversity at Fusarium head blight
114
Materials and methods
120
Discussion
136
References
144
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