Cryobanking the Genetic Resource: Wildlife Conservation for the Future?Paul Watson, William V. Holt There is a growing awareness of a worldwide reduction in biodiversity and the urgent need to develop ways to redress the problem. This is the first major book devoted to the subject of genetic resource banking (GRB) and its role in preserving global animal biodiversity. In Cryobanking the Genetic Resource, expert contributors provide the non-specialist with an overview of the subject and the practical techniques associated with GRB. The book presents a basic introduction to the concepts, and then points the way to relevant literature for those who wish to develop practical applications. The first section deals with the potential contribution of GRB to biodiversity protection, while the second section offers an introduction to the basic cryobiology of gametes and embryos. In the first section, genetic considerations for planning interventions are discussed, together with disease control measures. This section explores related reproductive technologies necessary for the use of cryopreserved gametes and embryos, as well as a range of other issues such as ownership and security of stored material, and the size and location of cryopreserved material. The second section offers a thorough review of the literature on cryopreservation of spermatozoa, oocytes, and embryos. It gathers species into related groups for ease of reference, and does not assume extensive specialist knowledge so that newcomers to the field can make the best use of the available research. This book is an essential resource for zoologists and conservationists with an interest in genetic management and captive breeding, and also for students involved in biodiversity issues, conservation, and assisted reproduction. |
Contents
CHAPTER 3 | 23 |
CHAPTER 4 | 47 |
CHAPTER 5 | 69 |
CHAPTER 7 | 89 |
CHAPTER 10 | 95 |
CHAPTER 8 | 123 |
PART B Survey of the Literature on Cryopreservation | 141 |
CHAPTER 12 | 201 |
CHAPTER 14 | 267 |
CHAPTER 15 | 279 |
CHAPTER 16 | 309 |
CHAPTER 18 | 361 |
CHAPTER 20 | 409 |
MORRELL¹ AND J K HODGES2 | 411 |
CHAPTER 21 | 427 |
| 449 | |
Common terms and phrases
acrosomal integrity animals artificial insemination assessment Biol blastocysts boar bovine captive cells CHAPTER concentration conservation cooling rates cryo Cryobiology cryopreservation cryoprotectant culture deer diluent dilution disease DMSO domestic cat donor effects egg yolk ejaculates electroejaculation embryo cryopreservation embryo transfer equilibration female fish follicles follicular freezing and thawing frozen semen frozen-thawed gametes genetic diversity genetic resource banking germplasm giant panda glycerol gonadotrophins gorilla Holt hormone human immature oocytes Johnston koala liquid nitrogen Loskutoff males marmoset marsupial medium method Mohor Mohor gazelle monkey morulae mouse embryos mouse oocytes non-domestic oestrus oocytes osmotic ovarian ovaries ovulation pellets Pope populations post-thaw motility potential pregnancy preservation primates procedure PrOH protocols Rall recovery Reprod samples semen collection slow cooling solution species sperm cryopreservation sperm motility spermatozoa stage storage straws studies successful sucrose survival techniques temperature Theriogenology tissue ungulates viability vitrification vitro fertilization vitro maturation Watson WHITTINGHAM wild wildlife WILDT Zool