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acetic acid acid solution added alcohol alkaline alkaloids ammonia ammonium amount analysis antimony apparatus aqueous arsenic barium boiling boric acid bromine butter c.c. of water calcium carbonate cent centimetres Chem chemical Chim chloride chloroform colour containing cooled copper Detection Determination dilute dissolved distilled drops Estimation ether evaporated examination excess experiments extract fatty acids ferric filtered filtrate flask gives grammes heated hydrochloric acid ignited insoluble Iodine value iron Journ liquid litre margarine matter mercury metal method methyl methyl orange milk milligramme millimetres minutes mixed mixture nitric acid nitrogen obtained oxalate oxide permanganate Pharm phosphoric acid platinum potash potassium iodide powder precipitate present Public Analysts pure quantity reaction reagent residue salts sample separated shaken silver sodium hydroxide soluble specific gravity standard starch substance sulphate sulphide sulphuric acid temperature titrated treated tube urine volume Volumetric washed water-bath weighed whilst yield Zeit zinc