Gene Cloning: An IntroductionThird edition. This concise and accessible introduction to gene cloning assumes almost no background knowledge. |
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Page 77
... ampicillin into a form that is non - toxic to the bacterium , and a different set of genes that code for enzymes that detoxify tetracycline . Uptake of pBR322 can be detected because the E. coli cells are transformed from ampicillin and ...
... ampicillin into a form that is non - toxic to the bacterium , and a different set of genes that code for enzymes that detoxify tetracycline . Uptake of pBR322 can be detected because the E. coli cells are transformed from ampicillin and ...
Page 81
... ampicillin , but sensitive to tetracycline ( ampRtet ) . ( a ) Colonies on ampicillin medium ( b ) Replica plating Wooden block Touch surface Touch surface Colonies on ampicillin medium ( c ) Amp tet colonies grown on tetracycline ...
... ampicillin , but sensitive to tetracycline ( ampRtet ) . ( a ) Colonies on ampicillin medium ( b ) Replica plating Wooden block Touch surface Touch surface Colonies on ampicillin medium ( c ) Amp tet colonies grown on tetracycline ...
Page 82
... ampicillin medium and incubated until colonies appear ( Fig . 5.7a ) . All colonies will consist of transformants ( remember , untransformed cells are amp so do not produce colonies ) , but only a few will contain recombinant pBR322 ...
... ampicillin medium and incubated until colonies appear ( Fig . 5.7a ) . All colonies will consist of transformants ( remember , untransformed cells are amp so do not produce colonies ) , but only a few will contain recombinant pBR322 ...
Contents
Preface | 1 |
plasmids and bacteriophages | 13 |
Purification of DNA from living cells | 25 |
Copyright | |
12 other sections not shown
Common terms and phrases
Adaptors agar amino acid ampicillin animal antibiotic antibody B-galactosidase bacterial cells bacteriophage bacterium BamHI base pairing biotechnology blunt ended carried cDNA cell extract centrifugation Chapter cleavage cleave cloned gene cloning vectors coded coli coli cells colonies contain copy number cosmid culture density digestion DNA fragments DNA ligase DNA sequencing double-stranded EcoRI electrophoresis enzyme expression vector Figure gene cloning gene cloning experiment host cell hybridization probing insulin intron labelled lacZ leu2 ligation lysed medium method mitochondrial Molecular Biology mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide organisms packaging phage DNA phage particles plant cells plaques plasmid DNA polylinker polymerase polynucleotide preparation produced promoter pUC8 purified recognition sequences recombinant DNA recombinant DNA molecule recombinant protein replication restriction endonucleases restriction sites resulting segment specific sticky ends strand structure synthesis T-DNA techniques tetracycline Ti plasmid transformed translation product trpA types unique restriction virus vitro yeast zein