Evidence for Functional Conservation, Sufficiency, and Proteolytic Processing of the CLAVATA3 CLE DomainUniversity of Michigan., 2006 |
Common terms and phrases
active CLE peptide alleles amino acids analysis anti-GST antibodies antibodies apices Arabidopsis shoot meristem Arabidopsis thaliana assay AtCLE25 AtCLV3 BY-2 cells BY-2 culture media C-terminal carpels carpels per flower cauliflower extracts CLAVATA3 CLE domain CLE family CLE motif CLE proteins CLE1 CLE6 CLE8 cleavage CLV signaling CLV1 CLV2 CLV3 CLE CLV3 expression CLV3 processing activity conserved cross-complementation detected with anti-GST E.coli expression encodes extracellular Figure flower meristems fluorescence genetic genomic GST-mCLE1 GST-mCLV3 GST-tagged His-mCLV3 incubated for 2h incubated with cauliflower indicated kinase ligand mature CLV3 mCLV3 MtCLE35 mutants myc-CLV3 NCBI null alleles p1 and p2 PCLV3 phenotypes Plant Cell polypeptide potato cyst nematode primers protease protein extracts Proteins were separated proteolysis proteolytic regulated residues SDS-PAGE SDS-PAGE and detected Selaginella separated by SDS-PAGE sequence shoot and flower signal peptide specific stem cell substrate suggest TaCLE30 tissues Tobacco BY-2 cells transformed into c/v3-1 transgenic triton variable domain vector vitro ZmESR1g1