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Isolation Characterization and Expression of Novel Protein Kinase Genes from Arabidopsis
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amino acid antisense Arabidopsis thaliana Atpk1 protein kinase Atpkl Atpkl and Atpkl Atpkl cDNA Atpkl expression Atpkl gene Atpkl mRNA Atpkl promoter autophosphorylation baculovirus Biol Chem blot analysis Brassica catalytic domain cdc2 cDNA Cell Biol conserved construct containing cytoplasmic data not shown detected doublet EcoRI enzymes eukaryotic expressed in insect function genomic DNA GUS activity Hanks higher plants Hunter I I I I Immune-complexes indicated insect cells intron isolated kanamycin kinase assay Kozma lysine metabolism mitogen-activated protein kinase Molecular cloning mutation Natl Acad nucleotides oligonucleotide PCR products peptide phosphatase phosphopeptide phosphorylation Plant Cell Plant Physiol polysome probe Proc protein kinase activity protein kinase gene protein kinase homologs protein phosphorylation region regulation regulatory domain residues ribosomal protein ribosomal S6 protein S6 protein kinase SDS-PAGE seedlings serine/threonine kinase Sf9 cells signal transduction Southern blot structure substrate transcription transgenic plants Trends Biochem