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Culture and Use of Primary and Clonal Neural Cells
Generation of Astrocyte Cultures from Normal and Neoplastic
Isolation of Astrocytes and Schwann Cells for Culture
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acid aggregates astrocytes biochemical Biol Bottenstein bovine brain cells Brain Res buffer Ca2+ calf serum carbachol cell cultures cell lines cell suspension cell types cellular centrifugation collagen concentration containing coverslips culture medium days in vitro density dextran differentiation diluted dissected dissociated DMEM dorsal embryo enzyme ethanol explants fetal fibronectin filter flask fluorescent forceps GFAP glia glial cells glioma glioma cells gradient growth factor hormone incubated injected isolated labeled layer Maximow method mg/ml microcarriers microscope monolayer mouse myelin Natl neonatal neural neurite Neurochem neurons Neurosci O. D. Laerum oligodendrocytes Pasteur pipette pellet petri dish phenotype pituitary plastic plated prepared primary cultured procedure protein rat brain receptor release removed retina rinsed RMAs S. M. Crain Schwann cells Sigma solution sterile studies sucrose techniques tion tissue culture Trypan blue trypsin tube tumor vitro vivo washed xg/ml