Plasmid Interactions and Gene Amplification in Bacterial Cells |
Contents
Results | 22 |
Involvement of fin0 inactivation in determination | 28 |
Analysis of plasmid DNA from a donor strain | 31 |
6 other sections not shown
Common terms and phrases
agarose gel Agarose gel electrophoresis ampicillin resistance amplification frequency amplification of NR1 amplified NR1 antibiotic concentrations antibiotic resistance antibiotic resistance genes Apr transconjugants bacterial Bacteriol bands cam DNA cells containing NR1 cells cultured Chapter chromosome clones cointegrate formation concentrations of neomycin containing amplified plasmids copy number cotransfer frequency cultured in medium DNA sequences DNA was isolated donor EcoRI fragment Escherichia coli factor Figure fragment G gene amplification genetic high concentration homologous recombination host isolated from cells lane lification mating mutants neo/kan determinants neo/kan gene neomycin per ml novel fragment NR1 derivatives plasmid DNA plasmid DNA isolated plasmid NR1 plates containing 200 plating efficiency Proteus mirabilis PstI r-determinant of NR1 recombination sites region replication Restriction endonuclease restriction endonuclease digestion RTF-Tc SalI fragment selection sequence homology spread onto plates stationary-phase strain streptomycin subcultured tandem duplication tandem r-determinants thymine trans transposon unamplified undergo amplification wild type NR1 µg/ml