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Common terms and phrasesaffinity agar amino acids amplified annealing antibody antigen bacterial beads Biacore Biol buffer cDNA CDR3 cells Centrifuge Chapter clones codon coli concentration containing culture described in Protocol digested dilution displayed protein DNA ligase DNA polymerase dNTPs domain electroporation ELISA elution encoding England Biolabs enzyme Equipment and reagents filamentous phage fragments gene repertoire helper phage immobilized Incubate infection IPTG ligands ligation linker membrane method microcentrifuge tube microtiter plates monovalent mutagenesis mutations NaCl Natl oligonucleotide overnight PCR product pellet peptide phage antibodies phage binding phage display phage library phage particles phagemid phagemid library phagemid vectors pill plasmid primer protease purified pVIII Qiagen random reaction reagents q receptor recombination Resuspend room temperature round of selection scFv scFv gene screening sequence shaking solution specific step sterile supernatant target template tissue ug/ml V-genes variants vivo VL gene Wash wild-type XLl-Blue References to this bookFrom other books
From Google ScholarSignal sequences directing cotranslational translocation expand ...Daniel Steiner, Patrik Forrer, Michael T Stumpp, Andreas Plückthun - 2006 - Nature Biotechnology The Use of Phage-Displayed Peptide Libraries to Develop Tumor ...Lauren RH Krumpe, Toshiyuki Mori - 2006 - International Journal of Peptide Research and Therapeutics Efficient Selection of DARPins with Sub-nanomolar Affinities using ...Daniel Steiner, Patrik Forrer, Andreas Plückthun - 2008 - Journal of Molecular Biology Preferred substrate sequences for transglutaminase 2: screening ...Kiyotaka Hitomi, Miyako Kitamura, Yoshiaki Sugimura - Amino Acids Bibliographic information
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