Methods in Systems Biology, Volume 500Daniel Jameson, Malkhey Verma, Hans Westerhoff Systems biology is a term used to describe a number of trends in bioscience research and a movement that draws on those trends. This volume in the Methods in Enzymology series comprehensively covers the methods in systems biology. With an international board of authors, this volume is split into sections that cover subjects such as machines for systems biology, protein production and quantification for systems biology, and enzymatic assays in systems biology research.
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Contents
Preface | xxiii |
Methods in Enzymology | xxv |
Section One | 1 |
Machines For Systems Biology | 13 |
Nucleic Acids and Systems Biology | 77 |
Protein Production and quantification for systems biology | 111 |
Enzymatic Assays in Systems Biology Research | 213 |
Sample Preparation in Metabolic Studies | 259 |
Mathematical Modelling in Systems Biology | 353 |
Understanding Systems Biology | 435 |
Managing Systems Biology Data Models and Research | 627 |
Author Index | 673 |
| 701 | |
Colour plate | 717 |
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Common terms and phrases
activity amino acids analyzed annotation applied approach assay Biochem Bioinformatics Biol biomass Biotechnol broth buffer calculated cDNA cell cellular centrifuge cerevisiae chemical chemostat coefficients coli complex components compound balance concentration constraints culture database Edited EFMs enzyme enzyme kinetics example experimental extraction flux flux balance analysis function gene expression genetic genome genome-scale metabolic glucose glycolysis growth Hofmeyr incubate interactions intracellular labeling Manchester mass spectrometry matrix measured megaterium metabolic models metabolic networks metabolites metabolomics method module mol/h molecular molecules mRNA noise optimal organism Palsson parameters pathway peptides phase plasmid production profiling protein proteomics protocol QconCAT quantification reaction reconstruction regulation analysis Saccharomyces cerevisiae sample SBML sequence signal SILAC solution specific spectroscopy standard structure studies substrate supernatant synthesis systems biology tion transcription trehalose tube vivo Vmax VOLUME Westerhoff yeast