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acid activity acute adult animals antigen atom bacteriophage Biol Bled Bled blood brain Brunhilde bulbar carbon cells cent centrifuged Chem clinical complement complement-fixing concentration configuration cotton rats Coxsackie virus developed dilution disease dose effect encephalitis epidemic experimental experiments factor fluid folinic acid Foundation for Infantile gamma globulin glycolysis glyoxalase hamsters helix hydrogen bonds immunity Immunol Infantile Paralysis infection inoculated intracerebral isolated Lansing Lansing poliomyelitis Lansing strain Lansing virus lesions Louis encephalitis lymph nodes Melnick method mice monkeys mouse muscle myelitis nerve neutralization tests neutralizing antibodies NH4+ normal observed obtained occurred pancreatic paralytic patients period phage phosphate pleated sheet polio poliomyelitis virus polypeptide pool positive prepared present Proc proteins reaction reported residue respiration respiratory sedimentation sera serum spinal cord strain of poliomyelitis structure substantia nigra suspension Table tion tissue culture titer ultracentrifuge vaccinated viruses vitamin weeks Y-SK