Chromatography: Fundamentals and applications of chromatography and related differential migration methods - Part A: Fundamentals and techniques (Google eBook)
Elsevier, Apr 16, 2004 - Science - 584 pages
Chromatography has emerged as the most important and versatile analytical method. The book is not only an updated version of Heftmann's classical text, but it covers areas of future importance, such as microfluidics and computer resources. Under his experienced guidance, authorities in each field have contributed their practical experience to an integrated treatment of modern micro analysis.
In Part A the theoretical basis of individual separation methods is explained and the technical aspects are illustrated. It includes the theory of gas and liquid chromatography as well as specific chromatographic techniques, such as size-exclusion, planar, ion, and affinity chromatography as well as various electrokinetic separation techniques. Microfluidics are covered for the first time and useful sources of analytical instruments are listed and evaluated.
1. Each chapter written by an authority
2. Thorough treatment of the theoretical basis of separation methods
3. Practical guide for performing analyses
What people are saying - Write a review
acetonitrile acid adsorption afﬁnity Anal analysis analytes anions applications Biochem buffer capillary column Capillary Electrophoresis cation Chem chemical chiral Chromatogr complex components compounds concentration detection detector devices diameter difﬁcult diffusion coefﬁcient Dionex dipole effect efﬁciency efﬂuent electrokinetic eluent elution ﬁeld ﬁrst ﬂow ﬂow-rate ﬂuorescence fraction gas chromatography gradient groups HPLC identiﬁcation increase inﬂuence injection injector instrument interactions ion-exchange ionization layer LC–MS ligand linear liquid chromatography mass spectrometry methanol methods microfabricated microﬂuidic mobile phase mobile-phase mode modiﬁed molecular molecules Nyiredy on-line open-tubular OPLC optimization P.G. Righetti packed column parameters particles peak peptides performance planar chromatography plate height polar polymeric polymers pressure proﬁle proteins pump puriﬁcation range resolution response retention factor reversed-phase sample selectivity separation signiﬁcant signiﬁcantly Silica size-exclusion solubility solute solvent solvent system sorbents speciﬁc stationary phase surface techniques temperature typical velocity volume