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amplitude pulses anticoagulated artificial materials biomaterials Biomer blood contact blood flow blood samples cardiopulmonary bypass cell holder coated counting efficiency curve data collection DC baseline decreased detection detector fibers developed diameter embolization erythrocytes ex vivo experiments fibrinogen filter Filtration Experiments glass flow cell hematocrit hemolysis heparin heparin drip illuminated volume implanted increase input laser intensity laser light scattering latex particles Lelah light scattering apparatus measured method microemboli minutes of blood ml/min Nalgene neutral density filters number of emboli observed optical fibers oscilloscope output oxidized polyethylene particle concentration particle passing particle size particle suspension particles in water peak photomultiplier tube plasma PMT power supply pore power supply voltage pulsatile flow pulse conditioning circuit pulse dampening chamber Pulse height analysis rinsed roller pump scattered intensity scattering volume silicone rubber spectra surface test material thromboembolism thrombogenicity thrombus deposition thrombus formation Tyrodes solution valves vitro vitro system whole blood