Molecular Cloning: A Laboratory Manual, Volume 1

Front Cover
CSHL Press, 2001 - Science - 2344 pages
8 Reviews

The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity.
In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology.
Handsomely redesigned and presented in new bindings of proven durability, this three–volume work is essential for everyone using today’s biomolecular techniques.
The opening chapters describe essential techniques, some well–established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small.
These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing.
The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein–protein interactions.
The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information.

As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.
  

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LibraryThing Review

User Review  - iayork - LibraryThing

the BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review

Review: Molecular Cloning: A Laboratory Manual

User Review  - Adrianna - Goodreads

Important book for someone in molecular biology field Read full review

Contents

INFORMATION PANELS
1-109
Chapter 9
1-117
Chapter 12
3
Preparation of Radiolabeled DNA and RNA Probes 9 1
9
Chapter 10
10
INFORMATION PANELS
58
INTRODUCTION
59
Chapter 13
69

Chapter 6
16-6
DMA Transfection Mediated by Lipofection 16 7
16-7
Chapter 8
16-8
INTRODUCTION
16-14
INFORMATION PANELS
16-51
Chapter 17
17-1
Introduction to Pulsedfield Gel Electrophoresis Protocols 1320 5 55
17-13
Isolation of DNA from 5 61
5
Purification of RNA from Cells and Tissues by Acid PhenolGuanidinium 7 4
7
Assay for Luciferase in Extracts of Mammalian Cells 17 42
2
s Ecdysone as Regulator of Inducible Gene Expression in Mammalian Cells 17 71
30
Chapter 18
18-1
Chapter 4
18-4
Working with Highcapacity Vectors 4 1
4
INFORMATION PANELS
59
INFORMATION PANELS
75
Appendices
85
INFORMATION PANELS
1-94
INFORMATION PANELS
1-107
PROTOCOLS
72
DNA Sequencing 12 1
12
Chapter 11
R-11
INTRODUCTION
R-20
1
1-1
1
1-13
Chapter 2
2
Chapter 2
12
Chapter 14
14
24
23
7
29
TECHNICAL COMMUNICATION
41
Removal of Small Fragments of Nucleic Acid from Preparations of Plasmid DNA by 1 82
82
HARDWARE
93
Forcing 117
117
ANALYSES
173
1
517
Copyright

Common terms and phrases

Popular passages

Page 12-20 - MJ: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.
Page 16 - The candela is the luminous intensity, in a given direction, of a source that emits monochromatic radiation of frequency 540 x 1012 hertz and that has a radiant intensity in that direction of (1/683) watt per steradian.
Page 17-2 - Science is built up with facts, as a house is with stones. But a collection of facts is no more a science than a heap of stones is a house.
Page 23 - Chirgwin, JM, Przybyla, AE, MacDonald, RJ, and Rutter, WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18, 5294-5299.
Page 12-20 - Harlow E. and Lane D. 1988. Antibodies: A laboratory manual Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Page R-9 - Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase.
Page 27 - Yanisch-Perron, C., Vieira, J., and Messing, J. (1985). Improved M13 Phage Cloning Vectors and Host Strains: Nucleotide Sequences of the M13mpl8 and PUC19 Vectors.
Page 27 - Smith, DB, and Johnson, KS (1988). Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67, 31-40.
Page 16-64 - Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

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About the author (2001)

Sambrook was a senior staff scientist at Cold Spring Harbor Laboratory from 1969-1985. He is Professor of Research at the Peter MacCallum Cancer Centre, Melbourne.

Bibliographic information