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absorption acid adaptation air saturation anaerobic autoxidation bicarbonate blood of Ch Bohr effect buffer capacity carbamate carbon dioxide carbonic anhydrase CD CD CD CN chironomid Chironomus blood Chironomus haemoglobin Chironomus larvae CN CD CN CN decrease demonstrated determined dialysed dilution equilibration evacuation experimental experiments external oxygen pressure Figure fluid function Gastrophilus greater haeme haeme-haeme interaction haemoglobin haemoglobin content Haldane effect HARNISCH Helix pomatia higher oxygen imidazole increase indicate iron content KEILIN and WANG larvae of Ch low oxygen concentrations manometers MANWELL measured metabolism methaemoglobin formation molecular obtained oxygen affinity oxygen capacity oxygen consumption oxygen equilibrium curve oxygen shortage oxygen tensions oxygen transport OxyHb partial pressures periods pH effect pH values phase pigment pigment concentration plumosus protein reaction vessel reduced respiration significant solution species spectrophotometric Table temperature thummi thummi blood tion tonometer tubes variation walshe Warburg Winkler titrations WOLVEKAMP