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acrosome reaction bioassay Biol blastocyst Ca2+ catheter cleavage clinical clomiphene citrate cortical granules culture cycle days diameter donor E2 levels Edwards eggs in vitro embryo transfer endometrial estradiol estrogen fertilization and embryo fertilization in vitro Figure follicles follicular aspiration follicular development follicular fluid follicular maturation follicular recruitment Gamete Garcia granulosa cells Gynecol hormone human in vitro human oocytes human sperm capacitation implantation incubator infertility insemination IVF-ET program laparoscopy Leeton LH surge Lopata luteal phase mammalian medium monitoring mouse needle normal Obstet oocyte maturation oocytes ovarian ovary ovulation patients pg/ml plasma membrane polyspermy pregnancy pregnancy rates preovulatory progesterone proteins protocols rabbit Reprod reproductive semen seminal plasma serum specific sperm capacitation sperm concentration sperm penetration sperm-egg spermatozoa spontaneous cycles Steptoe Steril stimulated cycles Testart treatment cycle Trounson tube ultrasound uterine uterus vitro fertilization Wolf Yanagimachi zona pellucida zona-free hamster egg