Animal transgenesis and cloning
Animal Transgenesis and Cloning is a concise, balanced introduction to this dynamic subject, covering key issues and current techniques currently used in animal transgenesis and cloning. Whilst providing the reader with the essentials of the subject, from the molecular basis of gene structure and function to therapeutic gene cloning in humans, social and ethical implications of this important area of research are also considered.
Written in a clear, accessible style, the book starts with an introduction to key molecular biology techniques and in particular, considers techniques used specifically for cloning animals and generating transgenic animals. Later chapters examine the diverse theoretical, technical and ethical issues raised by cloning and transgenesis in both animals and humans.
Animal Transgenesis and Cloning:
* Is an accessible, concise introduction to this dynamic field
* Discusses biological, technical and ethical issues related to cloning and transgenesis
* Provides an up-to-date account of the latest research methods and applications.
'This is a proposal for a book translation in a field that is clearly of importance and very topical. The idea of the book, from gene to transgenic animal, to its uses and abuses is sound and logical ...... It is probable that I would suggest (using) a book similar to the one proposed' Dr Janet Smith, University of Birmingham.
'Chapter one was a tour de force! Very concise and admirably clear ...... I was particularly impressed with the author's 2-page summary of eukaryotic gene regulation, which is no mean achievment!' Dr David de Pomerai, University of Nottingham.
'...... I felt that this is a well written, topical book. It covers a lot of ground ...... in my opinion the author manages to provide a satisfying amount of detail and a well focussed treatment of each topic. I like the logic of the book, the fact that it pulls together all of the issues into one readable and manageable text' Dr Brendan Curran, Queen Mary, University of London.
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From the gene to the transgenic animal
Techniques for cloning and transgenesis
Applications of cloning and transgenesis
3 other sections not shown
active allele antibodies bacteria biological Biotechnol cDNA cell types chromatin chromosome cloned animals complex Cre recombinase cytoplasm differentiated cells diseases DNA fragments donor cells efficient embryos enhance essentially experimental farm animals fertilization Figure foreign DNA foreign gene gene coding gene constructs gene expression gene of interest gene replacement gene therapy gene transfer gene trap genetic genome genomic DNA harbouring homologous recombination Houdebine human inactivated induce inhibit initiation codon injected integration intron known living organisms LoxP sequences mammals mechanisms method methylation microinjection microsatellites milk modified mouse mRNA mutation nuclear donors nuclear transfer nucleus obtained oligonucleotides oocyte organ stem cells origin of replication patients phenotypic plasmid pluripotent pluripotent cells possible promoter proteins protocol region reporter gene retroviral vectors ribozyme somatic cells specific sperm splicing targeted gene techniques telomeres therapeutic cloning transcription factors transfection transgenesis transgenic animals transgenic mice transposon vector contains viral genome virus vitro vivo