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Fine Structural Cellular and Mineral Changes
Sheaths and Reticular Two Patterns
A SEM Examination of QuickFrozen
20 micrometers Figure 24 hour regenerates 48 hour 72 hours arrows point artifact axially cut basal lamina biomineralization occurred broad headed arrows calcite calcoblast cap calcoblastic reticula calcoblastic sheath Cell types cellular processes extending cellular sheath chemically stabilized/critical point circular bodies cross buttressing cross sections crystallites cytoplasm distal Draeger echinoid electron lucent electron microscopy eleocytes epidermal cells Ep examined extending from calcoblasts extracellular freeze drying freezing frozen/freeze dried regenerates granular granulocytes growing microspines Heatfield and Travis hollow arrow Markel and Roser mature spines microspine tips microvilli millimeters mineral surface mitochondria nucleus old regenerating spine old spine regenerate organelles phagocytes plasma membrane regenerating spine tips reticulum of cellular samples Scale bar scanning electron scanning electron microscopy Sclerocytes sea urchin sections through cellular serial sections Shimizu and Yamada skeletogenesis solid arrows stabilized/critical point dried stereom stroma Strongylocentrotus purpuratus syncytium tertiary spines tissues transmission electron vacuolecytes vacuoles week old regenerates week old spine