CELL BIOLOGY ASSAYS: ESSENTIAL METHODS: ESSENTIAL METHODS (Google eBook)

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Kreitzer Geri
Butterworth-Heinemann, Nov 19, 2009 - Science - 298 pages
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This text provides comprehensive protocols essential methods across cell biology. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at all stages to embark on basic problems using a variety of technologies and model systems.



  • Provides researchers with solutions in lab environments
  • Features an array of essential methods, including endocytic pathways, membranes, mitochondria, and in vitro motility
  • Information on a plethora of technologies needed to tackle complex problems
  

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Contents

MEMBRANE PROTEIN TRANSLOCATION ASSAYS EXOCYTOSIS ENDOCYTOSIS ERGOLGI TRANSLOCATION THROUGH THE ER
1
LOADING PROTEIN INTO CELLS TO ASSESS PM FUNCTION
81
PROTEIN TRANSLOCATION BETWEEN CYTOPLASM AND COMPARTMENTS OTHER THAN PLASMA MEMBRANE
95
MEASURING INTRACELLULAR SIGNALING EVENTS USING FLUORESCENCE MICROSCOPY
137
IN SITU CELL ELECTROPORATION TO STUDY OF SIGNALING PATHWAYS
163
CYTOSKELETON ASSAYS
209
Index
281
Copyright

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About the author (2009)

A native of Chile, Dr. Julio E. Celis received his bachelor’s degree from the University of Chile in 1964, and then completed his Ph.D. in the United States at the University of Iowa. After his postdoctoral training at the Medical Research Council Laboratory of Molecular Biology in Cambridge, England, he went on to become an assistant professor at the University of Chile. Dr. Celis was named an associate professor in 1975 at Aarhus University, Denmark, and in 1987 became Chairman of the University’s Institute of Medical Biochemistry. He is also the Chairman of the Danish Centre for Human Genome Research and was recently elected Secretary General-elect of the Federation of European Biochemical Societies (FEBS) 1997. With nearly 180 publications to his credit, Dr. Celis’ specialized areas of research include molecular mechanisms of cancer, human 2D gel protein databases and their link to genome data, signal transduction, and the biology of human skin.

Married, with three children and one cat (Max William), Dr. Celis currently resides in Denmark where he holds among his many titles the chair to the Symposium and Prize Committee of the Danish Cancer Society, as well as the acting Chairman of the Nordic Molecular Biology Association (NOMBA). Dr. Celis is currently the Vice President of the European Molecular Biology Laboratory (EMBL) Council and leader of the Danish Delegation to the OECD Megascience Forum subgroup on Bioinformatics. He is also the European Union Observer to the International Nucleotide sequence database collaboration and a member of the EMBO Course Committee.

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