CELL BIOLOGY ASSAYS: ESSENTIAL METHODS: ESSENTIAL METHODS (Google eBook)

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Kreitzer Geri
Butterworth-Heinemann, Nov 19, 2009 - Science - 298 pages
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This text provides comprehensive protocols essential methods across cell biology. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at all stages to embark on basic problems using a variety of technologies and model systems.



  • Provides researchers with solutions in lab environments
  • Features an array of essential methods, including endocytic pathways, membranes, mitochondria, and in vitro motility
  • Information on a plethora of technologies needed to tackle complex problems
  

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Contents

MEMBRANE PROTEIN TRANSLOCATION ASSAYS EXOCYTOSIS ENDOCYTOSIS ERGOLGI TRANSLOCATION THROUGH THE ER
1
LOADING PROTEIN INTO CELLS TO ASSESS PM FUNCTION
81
PROTEIN TRANSLOCATION BETWEEN CYTOPLASM AND COMPARTMENTS OTHER THAN PLASMA MEMBRANE
95
MEASURING INTRACELLULAR SIGNALING EVENTS USING FLUORESCENCE MICROSCOPY
137
IN SITU CELL ELECTROPORATION TO STUDY OF SIGNALING PATHWAYS
163
CYTOSKELETON ASSAYS
209
Index
281
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