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Staining and Histochemical Methods General
Blocking and Enzyme Digestion Methods
0-5 per cent alcian blue alcian blue solution alcian blue staining aldehyde aldehyde fuchsin aqueous neutral red azure blocking Blue Blue Blue Blue Blue Negative Blue Negative Negative carboxyl groups cartilage celloidin cent acetic acid cent alcian blue cent aqueous neutral chloride Chondroitin sulphate clear and mount connective tissue connective tissue mucins counterstain De-paraffinize sections Dehydrate demonstration diastase diastase digestion distilled water dyes electrolyte enzyme epithelial mucins FIMLT following technique given mucin glycogen goblet cells haematoxylin heparan sulphate hexamine hyaluronic acid hyaluronidase keratan sulphate mast cell metachromasia metachromatic method minutes mucicarmine mucins stain natural or synthetic Negative Negative Negative neutral mucins neutral red non-sulphated acid mucins Nuclei periodic acid pH 2-5 alcian pH levels positive control section purple reaction reactive Schiff reagent sections and take sialidase sialomucin Spicer stain with alcian strongly sulphated mucins sulphate groups synthetic resin test section type of mucin umbilical cord Unaffected Blue weakly sulphated