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0N type 0N type mlg ability affinity influence affinity maturation affinity receptors Ag-induced Ag-specific anti-PC anti-u antibody APC function APC performance assays binding activity calcium cell activation cell lines cell transfectants stimulated Claflin clonal selection clones co-culture cross-linking data not shown density and affinity detectable double positive ELISA Figure FITC flow cytometry fluorescence gene germ line hapten Havran Heavy Chain higher affinity hybridoma immune response immunoglobulin Immunol increased Ionomycin isotype low affinity lower affinity lymphocytes mature B cells mediate mice migD mIgM mlg expression mlg receptor mlgD mlgD+ mlgM mlgM+ line mlgM+D+ mlgM|o mlgMmod molecules mutated in CDR2 PC-BGG PC-BSA PC-PM PC-PM stimulation PC-protein phosphocholine polysaccharide pulsed receptor affinity receptor density receptor internalization receptor number response to PC-PM signal transduction single positive soluble somatic hypermutation somatic mutation specific splenic B cells stained stimulated with PC-PM Streptococcus pneumoniae supernatants transfectants expressing unmutated