Antibody Affinity Maturation: Clonal Selection at the Level of the B Lymphocyte SurfaceUniversity of Michigan., 1994 |
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Page 20
... cloning of mutated VDJ genes from hybridoma DNA . A Cu constant region clone , B2 , consisting of a 9.6 kilobase genomic ... clones were designated respectively as pV1.4M.KNa and pV1.4M.KNb . These hybridomas have the 95N change plus a ...
... cloning of mutated VDJ genes from hybridoma DNA . A Cu constant region clone , B2 , consisting of a 9.6 kilobase genomic ... clones were designated respectively as pV1.4M.KNa and pV1.4M.KNb . These hybridomas have the 95N change plus a ...
Page 21
... clones , in addition to other changes in CDR2 , CDR3 , and framework regions ( Claflin et al . , 1989 ) . Because of the common CDR2 change , their Ig are each denoted type KN . To clone the rearranged VDJ from 239.10D7 and 206.5E11 , a ...
... clones , in addition to other changes in CDR2 , CDR3 , and framework regions ( Claflin et al . , 1989 ) . Because of the common CDR2 change , their Ig are each denoted type KN . To clone the rearranged VDJ from 239.10D7 and 206.5E11 , a ...
Page 22
... clones were screened initially for expression of mlg by a modified ELISA ( George et al . , 1993 ) . Microtiter ... clones for mlg using specific antibody , with direct flow cytometric analysis . If transfected clones displayed mixed ...
... clones were screened initially for expression of mlg by a modified ELISA ( George et al . , 1993 ) . Microtiter ... clones for mlg using specific antibody , with direct flow cytometric analysis . If transfected clones displayed mixed ...
Common terms and phrases
ability affinity maturation affinity receptors Ag-binding Ag-specific Ag:mlg interactions analyzed by flow anti-u antibody antigen APC function binding activity CaČ calcium calcium-binding dye CDR2 and CDR3 cell lines cell transfectants stimulated Claflin clonal selection clones co-culture concentration contour plots cross-linking data not shown density and affinity detectable double positive mIgM*D ELISA FACScan Figure FITC flow cytometry Fluo-3AM fluorescence Fluorescence data gene hapten Havran higher affinity HN mIgM hybridoma immune responses Immunol increased independent of isotype Intracellular Ca2+ fluxes Ionomycin isotype KN type lymphocytes mature B cells mean fluorescence mediating mice mIgD mIgDmod line mIgM mIgMio mIgMmod mlg expression mlg receptor molecules mutated in CDR2 PC hapten PC-BGG PC-OVA PC-PM stimulation phosphocholine polysaccharide pre-stimulation pulsed quantitative receptor density receptor internalization receptor number response to PC-PM sample signal transduction single positive somatic hypermutation somatic mutations specific splenic B cells stained stimulated with PC-PM ug/ml unmutated