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0N type 0N type mlg ability affinity maturation affinity receptors Ag-induced Ag-specific Ag:mlg interactions anti-PC anti-u antibody APC function assays binding activity Ca2+ fluxes calcium cell activation cell lines cell transfectants stimulated Claflin clonal selection clones co-culture cross-linking data not shown density and affinity detectable double positive ELISA expressing mlg Figure FITC flow cytometry fluorescence gene germ line hapten Havran Heavy Chain higher affinity hybridoma immune response immunoglobulin Immunol increased Intracellular Ca fluxes Ionomycin isotype levels of mlg low affinity lower affinity lymphocytes mature B cells mediate mice mlg expression mlg receptor mlgD+ mlgM+ line mlgM+D+ mlgM|o mlgMmod molecules PC-BGG PC-BSA PC-PM PC-PM stimulation PC-protein phosphocholine polysaccharide pulsed receptor affinity receptor density receptor internalization receptor number response to PC-PM signal transduction single positive soluble somatic hypermutation somatic mutation specific splenic B cells stained stimulated with PC-PM Streptococcus pneumoniae supernatants transfectants expressing unmutated