Antibody Affinity Maturation: Clonal Selection at the Level of the B Lymphocyte SurfaceUniversity of Michigan., 1994 |
Common terms and phrases
ability affinity maturation affinity receptors Ag-binding Ag-specific Ag:mlg interactions analyzed by flow anti-u antibody antigen APC function binding activity Ca² calcium calcium-binding dye CDR2 and CDR3 cell lines cell transfectants stimulated Claflin clonal selection clones co-culture concentration contour plots cross-linking data not shown density and affinity detectable double positive mIgM*D ELISA FACScan Figure FITC flow cytometry Fluo-3AM fluorescence Fluorescence data gene hapten Havran higher affinity HN mIgM hybridoma immune responses Immunol increased independent of isotype Intracellular Ca2+ fluxes Ionomycin isotype KN type lymphocytes mature B cells mean fluorescence mediating mice mIgD mIgDmod line mIgM mIgMio mIgMmod mlg expression mlg receptor molecules mutated in CDR2 PC hapten PC-BGG PC-OVA PC-PM stimulation phosphocholine polysaccharide pre-stimulation pulsed quantitative receptor density receptor internalization receptor number response to PC-PM sample signal transduction single positive somatic hypermutation somatic mutations specific splenic B cells stained stimulated with PC-PM ug/ml unmutated