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The Rationale for and Elements of Mammalian Cell Technology
Genetic Engineering in Mammalian Cells
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Acad adenovirus agitation amino acids amplified antigen base pairs Berg Biochem Biol carbohydrate cell adhesion cell culture cell growth cell lines cell surface cell types cellular Chem chromosome cloned containing copy number culture medium dissolved oxygen effect endoplasmic reticulum enhancer enzymes episomal extrachromosomal fermentor fibroblasts fibronectin Figure foreign gene fructose FS-4 cells galactose genetic genome glucose glutamine glycoproteins glycosylation Golgi growth factors growth rate hamster hormone host cell human increase infection insulin integrated intracellular Kruse lactate lysosomal mammalian cells marker membrane metabolism microcarrier culture molecular molecules monkey mouse cells Natl nutrient oligosaccharide oxygen concentration oxygen transfer perfused plasmid polypeptide Proc processing production promoter protein reactor receptor recombinant DNA replication residues retrovirus Sato secretory selectable sensors serum serum-free medium shear signal specific splice studies substrata substratum surface aerator SV40 virus synthesis thymidine kinase transcription transfected transformed utilization vessel viral viruses vitro