Caenorhabditis Elegans: Cell Biology and Physiology

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Joel H. Rothman, Andrew Singson
Academic Press, 2012 - Science - 458 pages
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The second part of an updated edition of the classic Methods in Cell Biology, Volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.



  • Diverse, up-to-date techniques covered will be useful to the broadening community of C. elegans researchers for years to come
  • Chapters written by leaders in the field
  • Tried and true methods deliver busy researchers a one-stop compendium of essential protocols
  

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Contents

Immunofluorescence Microscopy
35
Strategies and Applications
67
Modern Electron Microscopy Methods for C elegans
93
Culture and Manipulation of Embryonic Cells
153
Laser Microsurgery in Caenorhabditis elegans
177
Analysis of Cell Polarity in C elegans Embryos
207
Analysis of MembraneBound Organelles
239
C elegans Cell Cycle Analysis
265
Methods for Studying Programmed Cell Death in C elegans
297
Methods for Studying the DNA Damage Response in the Caenorhabdatis elegans Germ Line
321
Analysis of Aging in Caenorhabditis elegans
353
Analyses of C elegans Fat Metabolic Pathways
383
Electrophysiological Methods for Caenorhabditis elegans Neurobiology
409
INDEX
437
VOLUMES IN SERIES
449
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