Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 53
Page 1-6
... antibiotics is believed to interfere with their active transport into the cell ( for review , see Davies and Smith 1978 ) . Virtually all plasmid vectors in common use carry one or more of the antibiotic resistance genes described above ...
... antibiotics is believed to interfere with their active transport into the cell ( for review , see Davies and Smith 1978 ) . Virtually all plasmid vectors in common use carry one or more of the antibiotic resistance genes described above ...
Page 1-81
... antibiotic resistance marker encoded by the plasmid . To maximize the efficiency of transformation , the cells should be gently agitated ( 225 cycles / minute or less ) during the recovery period . 16. Transfer the appropriate volume ...
... antibiotic resistance marker encoded by the plasmid . To maximize the efficiency of transformation , the cells should be gently agitated ( 225 cycles / minute or less ) during the recovery period . 16. Transfer the appropriate volume ...
Page 1-87
... antibiotic resistance genes and an appropriate distribution of restriction enzyme cleavage sites . The DNA to be inserted and the purified plasmid DNA are digested with restriction enzymes that recognize sites located in only one of the ...
... antibiotic resistance genes and an appropriate distribution of restriction enzyme cleavage sites . The DNA to be inserted and the purified plasmid DNA are digested with restriction enzymes that recognize sites located in only one of the ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml