Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page xii
... Plasmid DNA 1.31 Rapid Disruption of Bacterial Colonies to Test the Size of Plasmids 1.32 LARGE - SCALE PREPARATIONS OF PLASMID DNA 1.33 Amplification of Plasmids in Rich Medium Harvesting and Lysis of Bacteria 1.34 HARVESTING 1.34 ...
... Plasmid DNA 1.31 Rapid Disruption of Bacterial Colonies to Test the Size of Plasmids 1.32 LARGE - SCALE PREPARATIONS OF PLASMID DNA 1.33 Amplification of Plasmids in Rich Medium Harvesting and Lysis of Bacteria 1.34 HARVESTING 1.34 ...
Page 1-22
... plasmid , the strain of E. coli , and the technique that is to be used subsequently to purify the plasmid DNA . Although it is impractical to give precise conditions for each combination of plasmid and host , the following general ...
... plasmid , the strain of E. coli , and the technique that is to be used subsequently to purify the plasmid DNA . Although it is impractical to give precise conditions for each combination of plasmid and host , the following general ...
Page 1-56
... DNA by gel electrophoresis . If all of the plasmid DNA has been converted from circular to linear molecules , adjust the salt concentration appropriately and add the second enzyme . At the same time , set up a separate pilot reaction ...
... DNA by gel electrophoresis . If all of the plasmid DNA has been converted from circular to linear molecules , adjust the salt concentration appropriately and add the second enzyme . At the same time , set up a separate pilot reaction ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml