Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 2-55
... teriophage vectors are suppressed only by supE ( e.g. , Pam3 ) and others only by supF ( e.g. , Sam7 and Sam100 ) . Since the presence of suppressors does not affect the growth of bacteriophage A vectors that do not carry amber ...
... teriophage vectors are suppressed only by supE ( e.g. , Pam3 ) and others only by supF ( e.g. , Sam7 and Sam100 ) . Since the presence of suppressors does not affect the growth of bacteriophage A vectors that do not carry amber ...
Page 2-124
... teriophage having EcoRI endonu- clease sites only in the nonessential region of the genome . Proc . Natl . Acad . Sci . 71 : 3927 . Rosenberg , S.M. 1985. EcoK restriction during in vitro packaging of coliphage lambda DNA . Gene 39 ...
... teriophage having EcoRI endonu- clease sites only in the nonessential region of the genome . Proc . Natl . Acad . Sci . 71 : 3927 . Rosenberg , S.M. 1985. EcoK restriction during in vitro packaging of coliphage lambda DNA . Gene 39 ...
Page 4-52
... teriophage specific for the F + and Hfr mating types of Escherichia coli K - 12 . Science 131 : 932 . Lyons , L.B. and N.D. Zinder . 1972. The genetic map of the filamentous bac- teriophage fl . Virology 49 : 45 . Marvin , D.A. and H ...
... teriophage specific for the F + and Hfr mating types of Escherichia coli K - 12 . Science 131 : 932 . Lyons , L.B. and N.D. Zinder . 1972. The genetic map of the filamentous bac- teriophage fl . Virology 49 : 45 . Marvin , D.A. and H ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml