Basic and Clinical Applications of Flow Cytometry: Proceeding of the 24th Annual Detroit Cancer Symposium Detroit, Michigan, USA - April 30, May 1 and 2, 1992Frederick A. Valeriote, Alexander Nakeff, Manuel Valdivieso The focus of this symposium was on the present and future capabilities of flow cytometry for both medical and biological applications in cancer. This technology began with quite modest instrumentation, with limited capabilities to answer biological questions. Today, both the clinical workhorses and the powerful multi-laser, multi-detector, sorting machinery, coupled with sophisticated computers and storage devices and the increasing storehouse of markers and dyes, are taking us to the limit and beyond in finding answers to the cause and cure of cancer. In the past, both normal hematopoietic tissue and leukemias have been the tissue samples of choice in the application of flow cytometry, and some of the most recent applications with these tissues are presented here. However, the book also discusses the increasingly sophisticated disaggregation techniques which allow investigators the possibility to train their lasers on solid tumors. Not only can we use flow cytometry with associated fluorescent markers to understand the biology of cancer, but also the wide array of existing and developing markers provides us with important diagnostic tools in the detection of cancer early in either the malignant or relapse process. And the field comes full circle, with the use of the technology for gene mapping and other genetic studies to unlock the basic malignant process. |
Contents
ACTIVATION AND PROLIFERATION OF PURIFIED HEMATO | 5 |
FLOW CYTOMETRIC PROLIFERATIVE FRACTION ANALYSIS | 13 |
MITOGENIC AND NONMITOGENIC INDUCTION OF LYMPHO | 33 |
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Basic and Clinical Applications of Flow Cytometry Frederick A Valeriote,Alexander Nakeff,Manuel Valdivieso No preview available - 1995 |
Common terms and phrases
activation antigen Biol bone marrow breast cancer c-myc Cancer Res carcinoma CD4+ T-lymphocytes CD8+ cell cycle cell lines cell proliferation cell sorting cellular chemotherapy chromosome clinical correlated cultures cyclins cytome cytotoxic debris detection diploid dissociation DNA analysis DNA aneuploid DNA content DNA diploid DNA flow cytometry DNA histograms DNA ploidy doxorubicin drug efflux enzymatic enzyme expression factors fetal cells flow cytometric analysis flow cytometry flow karyotype fluorescence fraction gene glutathione head and neck high-speed histograms human Immunol induced intracellular leukemia lymph lymphocytes lymphoma markers megakaryocytes method molecular monoclonal antibodies multiparameter murine normal nuclear matrix nuclei oncogene Oncology paraffin-embedded parameters patients peak phase phenotype ploidy principal component prognostic protein rare cell subpopulations RCS/Cy mice RCS5 mice receptor response S-phase S-phase fraction samples SCCHN signal solid tumors sorted cells specific staining stem cells stimulation studies T-lymphocytes techniques therapy tion tissue topoisomerase topoisomerase II tumor tumor cells vitro