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activity adsorption alanine racemase alkaloids amino acid analysis antibodies antisense aqueous bacteria batch berberine binding Biochem Bioeng Biol biological biomass biopolymer bioreactor Biotechnol Biotechnology broth catalase cell cultures cell lines Chem chemical chromatography cloned coefficient coli column complex components concentration Crystal Growth determined effect electrode elution enzyme ethanol factor fermentation Figure fragment function gene genetic glucose homologous HPLC immobilized increase inhibition interactions kinetics liquid mass transfer medium membrane metabolic metabolites method Microbiol molecular molecules mutant obtained optimal organic oxidase oxygen oxygen transfer parameters particles peptide peroxisomes plant cell cultures plasmid polypeptide predicted probes production protein proteinase pullulanase purification reaction reactor recombinant region residues sensor separation sequence shear solution solvent specific stationary phase strain structure studies substrate subunits synthesis Table techniques transcript transport trypsin tryptophan virus viruses yeast yield